Other

Part:BBa_K3552013:Design

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)


SccJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SccJ is a ribozyme or insulator which can eliminate the interference mediated by the input promoter. We used three different ribozymes to measure their different ability of improving the reaction of ptac promoter. We calculate the value and compare the gradient of the graph of fluorescent against concentration of IPTG.


Source

artificially synthesized

References